C20orf3/APMAP Monoklonaler Antikörper

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• KD/KO Validated

C20orf3/APMAP Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6C2

Kat-Nr. : 67464-1-Ig

Synonyme

C20orf3, 1C6C2, Adipocyte plasma membrane-associated protein, APMAP, BSCv

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  WB analysis using 67464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67464-1-Ig

  WB result of C20orf3/APMAP antibody (67464-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C20orf3/APMAP transfected HepG2 cells.

• 

  WB analysis using 67464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 67464-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67464-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67464-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67464-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, LNCaP-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Kaninchenlebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67464-1-Ig bindet in WB, IHC, ELISA C20orf3/APMAP und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C20orf3/APMAP fusion protein Ag29996
• Vollständiger Name: chromosome 20 open reading frame 3
• Berechnetes Molekulargewicht: 416 aa, 46 kDa
• Beobachtetes Molekulargewicht: 42-46 kDa
• GenBank-Zugangsnummer: BC003501
• Gene symbol: APMAP
• Gene ID (NCBI): 57136
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.