TMEM230 Polyklonaler Antikörper

TMEM230 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 21466-1-AP

Synonyme

C20orf30, HSPC274, TMEM230, UNQ2432/PRO4992

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 21466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21466-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21466-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21466-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21466-1-AP (TMEM230 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21466-1-AP (TMEM230 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21466-1-AP bindet in WB, IHC, IF, ELISA TMEM230 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM230 fusion protein Ag15080
• Vollständiger Name: chromosome 20 open reading frame 30
• Berechnetes Molekulargewicht: 183 aa, 20 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC011990
• Gene symbol: C20orf30
• Gene ID (NCBI): 29058
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The function of TMEM230/C20orf30 has not been widely studied, and is yet to be fully elucidated. TMEM230 is primarily a transmembrane protein of synaptic vesicles in neurons. Disease-linked TMEM230 mutants impair synaptic vesicle trafficking leading to Parkinson's disease. Two isoforms were produced by alternative splicing with predicted MW of 13 and 20 kDa. 13 kDa isoform accounts for >95% of the total protein isoforms(27270108).

Publikationen

Species	Application	Title
human	WB	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB,IHC	J Alzheimers Dis; TMEM230 Accumulation in Granulovacuolar Degeneration Bodies and Dystrophic Neurites of Alzheimer's Disease; Authors - Sandra L Siedlak
mouse	WB,IF	Exp Anim; TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models; Authors - Haiqing Zhang

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