C4BPA Polyklonaler Antikörper

C4BPA Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11819-1-AP

Synonyme

C4BP, C4BPA, Proline rich protein, PRP

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Galerie der Validierungsdaten

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  WB analysis of human blood using 11819-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11819-1-AP (C4BPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 11819-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 11819-1-AP (C4BPA Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 11819-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11819-1-AP (C4BPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11819-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11819-1-AP (C4BPA Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11819-1-AP bindet in WB, IHC, ELISA C4BPA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C4BPA fusion protein Ag2387
• Vollständiger Name: complement component 4 binding protein, alpha
• Berechnetes Molekulargewicht: 597 aa, 67 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC022312
• Gene symbol: C4BPA
• Gene ID (NCBI): 722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP alpha chain (C4BPA).

Publikationen

Species	Application	Title
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
human	IHC	Oncotarget; Hepatitis B virus X protein up-regulates C4b-binding protein α through activating transcription factor Sp1 in protection of hepatoma cells from complement attack.; Authors - Guoxing Feng
human	WB	BMC Cancer; The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.; Authors - Ping Zhao
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong

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