C5 Monoklonaler Antikörper

C5 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2G8C9

Rezensionen (1)

Kat-Nr. : 66634-1-Ig

Synonyme

C5, C5a, C5, C5b, 2G8C9, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5a anaphylatoxin

---

• 

  WB analysis of human plasma using 66634-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit serum using 66634-1-Ig

  rabbit serum were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66634-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of HepG2 using 66634-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66634-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5 (66634-1-Ig, Clone:2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, rabbit serum
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

66634-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA C5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C5 fusion protein Ag17882
• Vollständiger Name: complement component 5
• Berechnetes Molekulargewicht: 1676 aa, 188 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC113740
• Gene symbol: C5
• Gene ID (NCBI): 727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig
	ELISA	Virulence; Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infection; Authors - Simin Deng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Neil (Verified Customer) (05-10-2019)

Unfortunately the C5 antibody did not perform as well as I had hoped. Either the gel was not prepared properly, or the antibody bound indiscriminately to the gel.

• Applications: Western Blot,
• Cell Tissue Type: Synovial Fluid