C5 Monoklonaler Antikörper

C5 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2G8C9

Rezensionen (1)

Kat-Nr. : 66634-1-Ig

Synonyme

C5, Complement C5, complement component 5, CPAMD4

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Galerie der Validierungsdaten

• 

  WB analysis of human plasma using 66634-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66634-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66634-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human C5 (66634-1-Ig, Clone: 2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66634-1-Ig bindet in WB, IF, FC, ELISA C5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C5 fusion protein Ag17882
• Vollständiger Name: complement component 5
• Berechnetes Molekulargewicht: 1676 aa, 188 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC113740
• Gene symbol: C5
• Gene ID (NCBI): 727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Neil (Verified Customer) (05-10-2019)

Unfortunately the C5 antibody did not perform as well as I had hoped. Either the gel was not prepared properly, or the antibody bound indiscriminately to the gel.

• Applications: Western Blot,
• Cell Tissue Type: Synovial Fluid