C5aR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C5aR Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Rezensionen (1)

Kat-Nr. : 21316-1-AP

Synonyme

C5A, C5a R, C5aR, C5AR1, C5R1, CD88

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Galerie der Validierungsdaten

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  WB analysis using 21316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21316-1-AP (C5aR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21316-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21316-1-AP (C5aR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21316-1-AP (C5aR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21316-1-AP (C5aR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21316-1-AP

  Immunofluorescent analysis of HepG2 cells, using C5AR1 antibody 21316-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 21316-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5aR (21316-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, PC-3-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lebergewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Produktinformation

21316-1-AP bindet in WB, IHC, IF, FC, ELISA C5aR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C5aR fusion protein Ag15968
• Vollständiger Name: complement component 5a receptor 1
• Berechnetes Molekulargewicht: 350 aa, 39 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC008982
• Gene symbol: C5AR1
• Gene ID (NCBI): 728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C5aR, also named as CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

Publikationen

Species	Application	Title
	WB	Immunity; Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response.; Authors - Laura Surace
	WB	Theranostics; The hepatocyte-specifically expressed lnc-HSER alleviates hepatic fibrosis by inhibiting hepatocyte apoptosis and epithelial-mesenchymal transition.; Authors - Kun Zhang
human	IF	Sci Rep; Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth.; Authors - Fanny Chmilewsky
pig	WB	Sci Rep; Cardiac Depression in Pigs after Multiple Trauma - Characterization of Posttraumatic Structural and Functional Alterations.; Authors - M Kalbitz
human	WB	Cell Signal; The C5aR1 complement receptor: A novel immunomodulator of insulin action in skeletal muscle; Authors - Dinesh S Shah ; KO Validated
human	IF	Front Immunol; Complement Activation and Organ Damage After Trauma-Differential Immune Response Based on Surgical Treatment Strategy.; Authors - Ina Lackner

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Muhammad (Verified Customer) (12-25-2022)

one of the important receptor of complement system and this antibody helped a lot in our study to examine its role in regenerative medicine.

• Applications: Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: MSC's/Fibroblasts/DPSC's

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