C7orf10 Polyklonaler Antikörper

C7orf10 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21589-1-AP

Synonyme

Dermal papilla-derived protein 13, DERP13, EC:2.8.3.13, ORF19, Succinate--hydroxymethylglutarate CoA-transferase

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  WB analysis using 21589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21589-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21589-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21589-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 21589-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, HepG2-Zellen, Maus-Pankreasgewebe, Maus-Skelettmuskelgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

21589-1-AP bindet in WB, IHC, ELISA C7orf10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C7orf10 fusion protein Ag16159
• Vollständiger Name: chromosome 7 open reading frame 10
• Berechnetes Molekulargewicht: 471 aa, 51 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC098318
• Gene symbol: C7orf10
• Gene ID (NCBI): 79783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C7orf10 is also named as SUGCT, DERP13 and belongs to the CaiB/BaiF CoA-transferase family. It may function as part of a multiunit enzyme complex (A), similar to the alpha-ketoglutarate dehydrogenase system, or as the second of two independent enzymes, the first of which decarboxylates alpha-ketoadipate to glutarate (B). This protein has 4 isoforms produced by alternative splicing with the molecular weight from 43 kDa to 51 kDa.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Life Sci; Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype.; Authors - Joanna Niska-Blakie
mouse	WB	bioRxiv; Odd-chain dicarboxylic acid feeding recapitulates the biochemical phenotype of glutaric aciduria type 1 in mice; Authors - Adam C Richert
human	WB	Front Aging Neurosci; A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations; Authors - Scott P Allen