CA13 Polyklonaler Antikörper

CA13 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16696-1-AP

Synonyme

CA XIII, CA13, Carbonate dehydratase XIII, Carbonic anhydrase 13, carbonic anhydrase XIII, CAXIII

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 16696-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16696-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 16696-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CA13 antibody (16696-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 16696-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16696-1-AP (CA13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

16696-1-AP bindet in WB, IHC, IF, ELISA CA13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA13 fusion protein Ag10086
• Vollständiger Name: carbonic anhydrase XIII
• Berechnetes Molekulargewicht: 262 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC052602
• Gene symbol: CA13
• Gene ID (NCBI): 377677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).

Publikationen

Species	Application	Title
mouse	IF	BMC Med Genomics; Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4-/- mouse; Authors - Jin-Young Koh