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Calbindin-D28k Polyklonaler Antikörper

Calbindin-D28k Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(39)

Kat-Nr. : 14479-1-AP

Synonyme

CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Cerebellum-Gewebe, humanes Hirngewebe, humanes Nierengewebe, Maus-Cerebellum-Gewebe, Mausnierengewebe, Ratten-Cerebellum-Gewebe, Rattennierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaus-Cerebellum-Gewebe, humanes Nierengewebe
Erfolgreiche Detektion in IF-FroMaus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14479-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA Calbindin-D28k und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Calbindin-D28k fusion protein Ag5861
Vollständiger Name calbindin 1, 28kDa
Berechnetes Molekulargewicht 30 kDa
Beobachtetes Molekulargewicht 28 kDa
GenBank-ZugangsnummerBC006478
Gene symbol Calbindin
Gene ID (NCBI) 793
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Calbindin-D28k antibody 14479-1-APProtokoll herunterladen
IHC protocol for Calbindin-D28k antibody 14479-1-APProtokoll herunterladenl
IF protocol for Calbindin-D28k antibody 14479-1-APProtokoll herunterladen
IP protocol for Calbindin-D28k antibody 14479-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
mouseIF

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
mouseIF

Autophagy

Heat-shock chaperone HSPB1 mitigates poly-glycine-induced neurodegeneration via restoration of autophagic flux

Authors - Ning Ding
mouseIF

Cell Death Differ

The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.

Authors - Fei Wang
ratIF

Autophagy

Autophagy and post-ischemic conditioning in retinal ischemia.

Authors - Biji Mathew

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Georgiana (Verified Customer) (09-11-2023)

Antibody labelled cell bodies and dendrites very clearly

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain
Calbindin-D28k Antibody Immunohistochemistry validation (1:1000 dilution) in mouse brain (Cat no:14479-1-AP)
FH

Ramzi (Verified Customer) (06-14-2023)

The brain vibratome sections were treated with Acid-Alcohol before the blocking solution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: brain tissue ''Vibratome ''
Calbindin-D28k Antibody Immunofluorescence validation (1:300 dilution) in brain tissue ''Vibratome '' (Cat no:14479-1-AP)
FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other
FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other