NDP52 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDP52 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Kat-Nr. : 12229-1-AP

Synonyme

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 12229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 12229-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12229-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12229-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12229-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12229-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12229-1-AP

  IP Result of anti-NDP52 (IP:12229-1-AP, 3ug; Detection:12229-1-AP 1:1000) with HeLa cells lysate 2150ug.

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12229-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NDP52 antibody (12229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 38 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

12229-1-AP bindet in WB, IP, IHC, IF, ELISA NDP52 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDP52 fusion protein Ag2866
• Vollständiger Name: calcium binding and coiled-coil domain 2
• Berechnetes Molekulargewicht: 446 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC015893
• Gene symbol: CALCOCO2
• Gene ID (NCBI): 10241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Publikationen

Species	Application	Title
mouse	WB	Cell; NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.; Authors - Zhenyu Zhong
human	WB	Mol Cell; PMID: 28965816; Authors - Shouheng Jin ; KO Validated
human	WB	Autophagy; TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression; Authors - Tai-Mei Zhang
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
mouse	WB	Nat Commun; Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.; Authors - Insup Choi
mouse	WB	Redox Biol; A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease; Authors - Yi Sun

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