Caldesmon Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caldesmon Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 20887-1-AP

Synonyme

CALD1, CAD, caldesmon 1, CDM

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  WB analysis using 20887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20887-1-AP

  WB result of Caldesmon antibody (20887-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caldesmon transfected HeLa cells.

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  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 20887-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Caldesmon Polyclonal antibody (20887-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HeLa-Zellen, HT-1080.Zellen, MDA-MB-231-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

20887-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Caldesmon und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caldesmon fusion protein Ag13676
• Vollständiger Name: caldesmon 1
• Berechnetes Molekulargewicht: 538 aa, 63 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC040354
• Gene symbol: CALD1
• Gene ID (NCBI): 800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).

Publikationen

Species	Application	Title
human	WB,IF	Cell Rep; Proline restores mitochondrial function and reverses aging hallmarks in senescent cells; Authors - Debanik Choudhury
human	WB	J Am Heart Assoc; Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.; Authors - Jiaqi Tang
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human	WB,IF	Front Physiol; PMID: 30108509; Authors - Iddrisu B Yabasin
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
mouse		bioRxiv; IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells; Authors - Victoria A Wong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (01-22-2024)

Excellent antibody. Results are better than expected. Works well in Co-IP as well as in western blotting

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293, A7R5, HTR8, Liver, Placenta, Aorta

Caldesmon Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in HEK293, A7R5, HTR8, Liver, Placenta, Aorta (Cat no:20887-1-AP)