CALHM2 Polyklonaler Antikörper

CALHM2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 19931-1-AP

Synonyme

CALHM2, FAM26B, Protein FAM26B

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  WB analysis using 19931-1-AP

  Mouse brain extracted membrane protein and total protein lysates were subjected to SDS PAGE followed by western blot with 19931-1-AP (CALHM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 19931-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 19931-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 19931-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 19931-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 19931-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CALHM2 antibody (19931-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

19931-1-AP bindet in WB, IHC, IF/ICC, ELISA CALHM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CALHM2 fusion protein Ag13909
• Vollständiger Name: calcium homeostasis modulator 2
• Berechnetes Molekulargewicht: 323 aa, 36 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC000039
• Gene symbol: CALHM2
• Gene ID (NCBI): 51063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CALHM2, also named as FAM26B, is a pore-forming subunit of a voltage-gated ion channel. It's a critical ATP-releasing channel that modulates neural activity and as a potential risk factor of depression. There're three isoforms of CALHM2 with calculated MW 36 kDa, 24 kDa and 22 kDa. It's about 32-36 kDa in western blot with membrane protein extraceted lysate.

Publikationen

Species	Application	Title
mouse,rat	WB	Cereb Cortex; P2X7 receptor-mediated depression-like reactions arising in the mouse medial prefrontal cortex; Authors - Wen-Jing Ren
mouse	IF	Brain Res Bull; Upregulation of Calhm2 in the anterior cingulate cortex contributes to the maintenance of bilateral mechanical allodynia and comorbid anxiety symptoms in inflammatory pain conditions; Authors - Xiaohui Li