CaMKII beta-Specific Polyklonaler Antikörper

CaMKII beta-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Kat-Nr. : 55218-1-AP

Synonyme

CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2, CAMK2B, CAMKB, CaMKII beta, CaMKII beta-Specific

---

Galerie der Validierungsdaten

• 

  WB analysis using 55218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55218-1-AP (CaMKII beta-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 55218-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55218-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 55218-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55218-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 55218-1-AP

  Immunofluorescent analysis of HEK-293 cells using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Maus-Cerebellum-Gewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55218-1-AP bindet in WB, IHC, IF CaMKII beta-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: calcium/calmodulin-dependent protein kinase II beta
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: NM_001220
• Gene symbol: CAMK2B
• Gene ID (NCBI): 816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CaMKII beta (Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAMK2B, CAM2 or CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals (PMID:22578382). CAMK2B has 8 isoforms produced by alternative splicing with the molecular weight 50-73 kDa. This antibody is specific to CAMK2B and can recognize all the isoforms except isofom 7.