CaMKII delta-Specific Polyklonaler Antikörper

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• KD/KO Validated

CaMKII delta-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 20667-1-AP

Synonyme

CaM kinase II subunit delta, CaMK II subunit delta, CAMK2D, CAMKD, CaMKII delta-Specific

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Galerie der Validierungsdaten

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  WB analysis using 20667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 20667-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20667-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20667-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20667-1-AP

  IP Result of anti-CaMKII delta-Specific (IP:20667-1-AP, 4ug; Detection:20667-1-AP 1:600) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 20667-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20667-1-AP (CaMKII delta-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20667-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20667-1-AP (CaMKII delta-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Skelettmuskelgewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20667-1-AP bindet in WB, IP, IHC, IF, ELISA CaMKII delta-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: calcium/calmodulin-dependent protein kinase II delta
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_001221
• Gene symbol: CAMK2D
• Gene ID (NCBI): 817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CaMKII delta, also named as CAMKD and CaMKII delta, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It can recognize most isoforms the gene encodes, from 50-72kd. It can recognize the isoform 2,3,4,9,11 of CAMK2D.

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.; Authors - Cui Ma
mouse	IHC	Cell Death Dis; miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.; Authors - Xudong Zhang
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang
rat	WB	J Pain Res; Proteomic Analysis of Emodin Treatment in Neuropathic Pain Reveals Dysfunction of the Calcium Signaling Pathway.; Authors - Peng Chen
human	WB	Oncol Lett; PMID: 30127991; Authors - Xiaoli Xu
mouse,rat	WB	Cell Mol Life Sci; JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes; Authors - Jiachen Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB (1:1000) and IF (1:200)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200