CAMKV Polyklonaler Antikörper

CAMKV Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 14788-1-AP

Synonyme

1G5, CAMKV, VACAMKL

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14788-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14788-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14788-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14788-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14788-1-AP

  IP Result of anti-CAMKV (IP:14788-1-AP, 4ug; Detection:14788-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of mouse brain using 14788-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14788-1-AP (CAMKV antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Mausmilzgewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14788-1-AP bindet in WB, IP, IF, ELISA CAMKV und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMKV fusion protein Ag6438
• Vollständiger Name: CaM kinase-like vesicle-associated
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-75 kDa
• GenBank-Zugangsnummer: BC000497
• Gene symbol: CAMKV
• Gene ID (NCBI): 79012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).

Publikationen

Species	Application	Title
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María Inmaculada (Verified Customer) (07-15-2022)

I used the antibody for Western Blot and it worked very well. First, we used it at a 1:500 dilution first, but it was super concentrated and obtained unspecific bands. Then, I used it at a 1:2500 dilution and obtained pretty clean bands for the protein. The signal was very intense with my system, so I think I could have even diluted it a bit more.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Mouse brain