CAP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2H3A10

Kat-Nr. : 68207-1-Ig

Synonyme

2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

---

• 

  WB analysis using 68207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HepG2 using 68207-1-Ig

  WB result of CAP1 antibody (68207-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAP1 transfected HepG2 cells.

• 

  WB analysis of pig lung using 68207-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 68207-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 68207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 68207-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 68207-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 68207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 68207-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 68207-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HepG2-Zellen, humanes Plazenta-Gewebe, Mauslungengewebe, Hausschwein-Lungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mauslungengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

68207-1-Ig bindet in WB, IHC, IF/ICC, ELISA CAP1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAP1 fusion protein Ag8876
• Vollständiger Name: CAP, adenylate cyclase-associated protein 1 (yeast)
• Berechnetes Molekulargewicht: 475 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC013963
• Gene symbol: CAP1
• Gene ID (NCBI): 10487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

Publikationen

Species	Application	Title
human		Int Immunopharmacol; Single-cell RNA sequencing reveals cell-cell communication and potential biomarker in sepsis and septic shock patients; Authors - Guilin Li
human	IF	Acta Neuropathol; Enhanced microglial dynamics and a paucity of tau seeding in the amyloid plaque microenvironment contribute to cognitive resilience in Alzheimer's disease; Authors - Nur Jury-Garfe
human	WB	J Neurochem; CPNE7 Regulates Amyloidogenesis Through CAP1-Dependent ADAM10 Translation; Authors - Jie Yang