CAPG Polyklonaler Antikörper

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• KD/KO Validated

CAPG Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 10194-1-AP

Synonyme

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Galerie der Validierungsdaten

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  WB analysis using 10194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10194-1-AP (CAPG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 10194-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10194-1-AP (CAPG Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 10194-1-AP

  WB result of CAPG antibody (10194-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells.

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  IP experiment of mouse heart using 10194-1-AP

  IP Result of anti-CAPG (IP:10194-1-AP, 4ug; Detection:10194-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10194-1-AP (CAPG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10194-1-AP (CAPG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10194-1-AP (CAPG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10194-1-AP (CAPG antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Rattenlungengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

10194-1-AP bindet in WB, IP, IHC, IF, ELISA CAPG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAPG fusion protein Ag0248
• Vollständiger Name: capping protein (actin filament), gelsolin-like
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC000728
• Gene symbol: CAPG
• Gene ID (NCBI): 822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publikationen

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.; Authors - Khositseth Sookkasem S
human	WB	J Proteome Res; Laser microdissection and two-dimensional difference gel electrophoresis reveal the role of a novel macrophage-capping protein in lymph node metastasis in gastric cancer.; Authors - Ichikawa Hiroshi H
human	WB	Clin Proteomics; Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients.; Authors - Lavanya Balakrishnan
human	WB	Inflammation; PMID: 25586482; Authors - Xin Ma
human	WB	Am J Respir Cell Mol Biol; Up-regulation of two actin-associated proteins prompts pulmonary artery smooth muscle cell migration under hypoxia.; Authors - Zhang Ruifeng R ; KD Validated
human	WB	Proteomics; Proteomics-based identification of two novel direct targets of hypoxia-inducible factor-1 and their potential roles in migration/invasion of cancer cells.; Authors - Liao Shi-Hua SH