CAPRIN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CAPRIN1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

63

Rezensionen (3)

Kat-Nr. : 15112-1-AP

Synonyme

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105

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Galerie der Validierungsdaten

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15112-1-AP

  IP result of anti-CAPRIN1 (IP:15112-1-AP, 4ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.

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  IHC staining of human breast cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 15112-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15112-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of OC2 cells using 15112-1-AP

  IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.

• 

  IF Staining of HeLa using 15112-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of cochlear cryosections using 15112-1-AP

  IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with Cochlear cryosections by Dr. Emily Towers. CAPRIN1, purple; DAPI, blue.

• 

  FC experiment of NIH/3T3 using 15112-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (15112-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen, OC2-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 46 publications below
• IHC: See 2 publications below
• IF: See 23 publications below
• IP: See 4 publications below
• RIP: See 2 publications below

Produktinformation

15112-1-AP bindet in WB, RIP, IP, IHC, IF, FC, ELISA CAPRIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAPRIN1 fusion protein Ag7099
• Vollständiger Name: cell cycle associated protein 1
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC001731
• Gene symbol: CAPRIN1
• Gene ID (NCBI): 4076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Publikationen

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
human	WB	Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang ; KD Validated
human	WB,IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
human	WB,IF	Nat Immunol; Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.; Authors - Henan Xu
mouse	WB,RIP	Nat Commun; Fear extinction is regulated by the activity of long noncoding RNAs at the synapse; Authors - Wei-Siang Liau
	WB, IP	Neuron; CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.; Authors - Silvia De Rubeis

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katherine (Verified Customer) (10-31-2023)

The antibody worked well with 1:5000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T

FH

Lisa (Verified Customer) (02-20-2020)

The antibody works very well, giving specific bands in WB and a very good staining in IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: WB:1:2500 - IF:1:100
• Cell Tissue Type: Fibroblasts, lymphoblastoid cell line

CAPRIN1 Antibody Western Blot,Immunofluorescence, validation (WB:1:2500 - IF:1:100 dilution) in Fibroblasts, lymphoblastoid cell line (Cat no:15112-1-AP)

FH

Paul (Verified Customer) (04-30-2019)

Product works very well for WB, IP and IF using rat brain tissue samples.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Rat brain

CAPRIN1 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:2000 dilution) in Rat brain (Cat no:15112-1-AP)

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