CARD14 Polyklonaler Antikörper

CARD14 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10400-1-AP

Synonyme

BIMP2, CARD14, Carma 2, CARMA2

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• 

  WB analysis of HeLa using 10400-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10400-1-AP (CARD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

10400-1-AP bindet in WB, ELISA CARD14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CARD14 fusion protein Ag0628
• Vollständiger Name: caspase recruitment domain family, member 14
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 113-140 kDa
• GenBank-Zugangsnummer: BC018142
• Gene symbol: CARD14
• Gene ID (NCBI): 79092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARD14/CARMA2 is a novel caspase recruitment domain-containing protein 14 and is also a membrane-associated guanylate kinase (MAGUK) family member. CARD domain is a protein-binding element that mediates the assembly of CARD-containing proteins into apoptosis and NF-kappaB signaling complexes, which has been implicated in inflammatory disorders. CARD14 activates NF-kappaB signaling via interaction with BCL10. Defects in CARD14 are the cause of susceptibility to psoriasis type 2 (PSORS2), which is a common inflammatory disorder of the skin and other organs caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.

Publikationen

Species	Application	Title
mouse	WB	EMBO Rep; MALT1 targeting suppresses CARD14-induced psoriatic dermatitis in mice.; Authors - Elien Van Nuffel
human	WB	Oncogene; MALT1 is a critical mediator of PAR1-driven NF-κB activation and metastasis in multiple tumor types.; Authors - J Randall McAuley
human	WB	Biomedicines; CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells; Authors - Domien Vanneste
mouse	WB	Biochem J; CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation; Authors - Paul A O'Sullivan

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