CARM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CARM1 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (2)

Kat-Nr. : 55246-1-AP

Synonyme

CARM1, PRMT4

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 55246-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55246-1-AP (CARM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55246-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55246-1-AP (CARM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55246-1-AP

  WB result of CARM1 antibody (55246-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARM1 transfected MCF-7 cells.

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 55246-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 55246-1-AP (CARM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes malignes Melanomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55246-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CARM1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: coactivator-associated arginine methyltransferase 1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 63-66 kDa, 55 kDa
• GenBank-Zugangsnummer: NM_199141
• Gene symbol: CARM1
• Gene ID (NCBI): 10498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.

Publikationen

Species	Application	Title
human	WB	J Med Chem; Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA; Authors - Limei Ji
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
mouse	WB	Front Cell Dev Biol; Osteocytes Enhance Osteogenesis by Autophagy-Mediated FGF23 Secretion Under Mechanical Tension.; Authors - Huiyue Xu
human	WB,CoIP	Cancer Cell Int; CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.; Authors - Chenxin Xu ; KD Validated
human	WB	RSC Adv; Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression; Authors - Guixin Wu
mouse	IF	J Reprod Immunol; Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development.; Authors - Zhicheng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

fatemeh (Verified Customer) (07-25-2022)

western blot was done on 50 ug of cell lysate. Blot was transfered using wet transfer method (300 mA for 90 min). 5% milk was used as blocking reagent. antibody dilution was 1:1000 and incubation with primary antibody was overnight at 4C. Multiple band between 75 and 50 kDa were detected. for IF, PFA was used for fixing the cells and 0.5% triton was used for permeabilization. antibody was diluted 1:200 and samples were incubated with primary ab for 1h at RT. a cytoplasmic signal for Carm1 was detected.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: mESC

CARM1 Antibody Western Blot,Immunofluorescence validation ( dilution) in mESC (Cat no:55246-1-AP)

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs