Caspase 1/P20 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 1/P20 Polyklonal Antikörper für WB, IHC, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (7)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1042)

Rezensionen (4)

Kat-Nr. : 22915-1-AP

Synonyme

CASP1, Caspase1, CASP 1, CASP-1, Caspase 1

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• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22915-1-AP

  WB result of Caspase 1/P20 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells.

• 

  IHC staining of human spleen using 22915-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 22915-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining using 22915-1-AP

  IF result of 22915-1-AP from publication with PMID: 31611559.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HepG2-Zellen, Jurkat-Zellen, PC-12-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 936 publications below
• IHC: See 159 publications below
• IF: See 175 publications below
• IP: See 2 publications below

Produktinformation

22915-1-AP bindet in WB, IHC, IF, IP, ELISA Caspase 1/P20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Ente, hamster, Hausschwein, Huhn, Hund, Kaninchen
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 1/P20 fusion protein Ag11143
• Vollständiger Name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Berechnetes Molekulargewicht: 404 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-47 kDa, 30 kDa, 35 kDa
• GenBank-Zugangsnummer: BC062327
• Gene symbol: Caspase 1
• Gene ID (NCBI): 834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize p45 procaspase and the mature fragment p20.

Publikationen

Species	Application	Title
human	WB	Cell Res; Signal-induced NLRP3 phase separation initiates inflammasome activation; Authors - Gonglu Zou
	WB	Mol Cancer; Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases; Authors - Qiang Wang
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
	IHC	J Hazard Mater; Perinatal exposure to PBEB aggravates liver injury via macrophage-derived TWEAK in male adult offspring mice under western diet; Authors - Wanyue Wang
human	WB	J Hazard Mater; Adverse outcome pathway-based approach to reveal the mechanisms of skin sensitization and long-term aging effects of chlorothalonil; Authors - Yung-Hsuan Cheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anne (Verified Customer) (04-01-2025)

It worked well in the Western Blot experiment.

• Applications: Western Blot

FH

Anne (Verified Customer) (01-31-2025)

It worked well in the Western Blot experiment.

• Applications: Western Blot

FH

Joseph (Verified Customer) (02-19-2020)

This antibody detected pro-caspase 1 and the p20 cleaved product in PDAC cell lines. I did not look for the p10 cleaved product.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PDAC cell lines

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain