Caspase 4/p20/p10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 4/p20/p10 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11856-1-AP

Synonyme

CASP 4, CASP4, Caspase 4, Caspase 4/p20/p10, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX

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Galerie der Validierungsdaten

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  WB analysis of SGC-7901 using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (CASP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (CASP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11856-1-AP (CASP4 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 11856-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below

Produktinformation

11856-1-AP bindet in WB, IHC, IF, ELISA Caspase 4/p20/p10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 4/p20/p10 fusion protein Ag2429
• Vollständiger Name: caspase 4, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43-48 kDa
• GenBank-Zugangsnummer: BC017839
• Gene symbol: CASP4
• Gene ID (NCBI): 837
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Publikationen

Species	Application	Title
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
human	WB	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
human	WB	Oral Dis; Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway; Authors - Bing-Jing Sun
human	WB	Chem Res Toxicol; Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation.; Authors - Xiaomin Xia
human	WB	Int J Mol Sci; PMID: 27827850; Authors - Wei-Ye Shi