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CASR Polyklonaler Antikörper

CASR Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 19125-1-AP

Synonyme

Calcium Sensing Receptor, CAR, Extracellular calcium-sensing receptor, FHH, FIH



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe, COLO 320-Zellen, HL-60-Zellen, Jurkat-Zellen, Mauslebergewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19125-1-AP bindet in WB, IHC, IF, ELISA CASR und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name calcium-sensing receptor
Berechnetes Molekulargewicht 121 kDa
Beobachtetes Molekulargewicht120-140 kDa
GenBank-ZugangsnummerNM_000388
Gene symbol Calcium Sensing Receptor
Gene ID (NCBI) 846
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASR, also named as GPRC2A, PCAR1, belongs to the G-protein coupled receptor 3 family. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. The activity of CASR is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo). The 60-65 kDa band detected by this antibody might represent a degradation product of CASR (PMID: 15718278).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CASR antibody 19125-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB,IHC,IF

J Ginseng Res

Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway

Authors - Mei-Li Lu
rat,humanWB,IF

Front Pharmacol

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.

Authors - Meili Lu
humanWB

Phytomedicine

Panaxytriol upregulates CYP3A4 expression based on the interaction of PXR, CAR, HSP90α, and RXRα.

Authors - Lingming Zhang
  • KD Validated
ratWB

Front Pharmacol

Effects of Sodium Ferulate on Cardiac Hypertrophy Are via the CaSR-Mediated Signaling Pathway.

Authors - Panpan Chen
ratWB

J Ethnopharmacol

Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats.

Authors - Beibei Chen
humanWB,IHC

Acta Pharmacol Sin

Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways.

Authors - Bo Yin