Caveolin-1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Caveolin-1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

CloneNo.

6C2B2

Kat-Nr. : 66067-1-Ig

Synonyme

CAV1, Caveolin 1, 6C2B2, BSCL3, CAV

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  WB analysis using 66067-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66067-1-Ig

  WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells.

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  IHC staining of human ovary tumor using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66067-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66067-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human skin cancer using 66067-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human skin cancer using 66067-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 66067-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 66067-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, PC-3-Zellen, Hausschwein-Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe, humanes Hautkrebsgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 11 publications below
• IP: See 3 publications below

Produktinformation

66067-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Caveolin-1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caveolin-1 fusion protein Ag8049
• Vollständiger Name: caveolin 1, caveolae protein, 22kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu
human	IF	Cancer Res; Inhibition of EGFR Overcomes Acquired Lenvatinib Resistance Driven by STAT3-ABCB1 Signaling in Hepatocellular Carcinoma; Authors - Beiyuan Hu
human	WB	Mol Metab; CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue.; Authors - Ningning Bai
human	WB	Cells; Blocking EREG/GPX4 Sensitizes Head and Neck Cancer to Cetuximab through Ferroptosis Induction; Authors - Aude Jehl
canine	WB,IP,IF	FASEB J; Lipid raft interacting galectin 8 regulates primary ciliogenesis; Authors - Jhan-Jhang Syu
mouse	IF	Food Chem Toxicol; α-Hemolysin suppresses osteogenesis by inducing lipid rafts accumulation in bone marrow stromal cells.; Authors - Guanqiao Liu