CBLB Polyklonaler Antikörper

CBLB Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 12781-1-AP

Synonyme

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12781-1-AP

  IP Result of anti-CBLB (IP:12781-1-AP, 4ug; Detection:12781-1-AP 1:300) with HEK-293 cells lysate 3680ug.

• 

  IHC staining of mouse colon using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 10x lens)..

• 

  IHC staining of mouse colon using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 40x lens)..

• 

  IHC staining of human kidney using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 12781-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBLB antibody (12781-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12781-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA CBLB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBLB fusion protein Ag3491
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Berechnetes Molekulargewicht: 982 aa, 109 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC032851
• Gene symbol: CBLB
• Gene ID (NCBI): 868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CBLB(E3 ubiquitin-protein ligase CBL-B) is also named as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; Cbl-b deficiency prevents functional but not phenotypic T cell anergy.; Authors - Trang T T Nguyen
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
human	FC	J Immunol; A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function.; Authors - Klarissa Hanja Stürner
mouse	WB,IF	J Cell Mol Med; NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.; Authors - Ze-Qing Pu
mouse	WB	Biomolecules; miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles.; Authors - Xin Yang