NCBP1/CBP80 Rekombinanter Antikörper

NCBP1/CBP80 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240012G8

Kat-Nr. : 83212-4-RR

Synonyme

NCBP1, NCBP 80 kDa subunit, NCBP, CBP80, 80 kDa nuclear cap-binding protein

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  WB analysis using 83212-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 83212-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83212-4-RR (NCBP1/CBP80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 83212-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 83212-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83212-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NCI-H1299-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83212-4-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA NCBP1/CBP80 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NCBP1/CBP80 fusion protein Ag0496
• Vollständiger Name: nuclear cap binding protein subunit 1, 80kDa
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC001450
• Gene symbol: NCBP1
• Gene ID (NCBI): 4686
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.