CBS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CBS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

96

Kat-Nr. : 14787-1-AP

Synonyme

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 14787-1-AP

  WB result of CBS antibody (14787-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected HEK-293 cells.

• 

  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14787-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14787-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14787-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14787-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 14787-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14787-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14787-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 14787-1-AP

  IP Result of anti-CBS (IP:14787-1-AP, 3ug; Detection:14787-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14787-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, Maushirngewebe, Maus-Kolongewebe, Mausnierengewebe, MCF-7-Zellen, Rattenhirngewebe, Rattenlebergewebe, Rattennierengewebe, SKOV-3-Zellen, THP-1-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 86 publications below
• IHC: See 14 publications below
• IF: See 15 publications below

Produktinformation

14787-1-AP bindet in WB, IP, IHC, IF, ELISA CBS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBS fusion protein Ag6437
• Vollständiger Name: cystathionine-beta-synthase
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61-63 kDa
• GenBank-Zugangsnummer: BC000440
• Gene symbol: CBS
• Gene ID (NCBI): 875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Publikationen

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
canine	IF	Nat Commun; Remote ischemic conditioning counteracts the intestinal damage of necrotizing enterocolitis by improving intestinal microcirculation.; Authors - Yuhki Koike
mouse	WB	Redox Biol; Cystathionine γ-lyase promotes estrogen-stimulated uterine artery blood flow via glutathione homeostasis.; Authors - Rachael Bok
human	WB	Redox Biol; Deletion of cystathionine-γ-lyase in bone marrow-derived cells promotes colitis-associated carcinogenesis.; Authors - Ketan K Thanki
mouse	WB,IF	Redox Biol; Endogenous H2S targets mitochondria to promote continual phagocytosis of erythrocytes by microglia after intracerebral hemorrhage; Authors - Xiaoling Yan

Verwandte Produkte

ubiquitin Polyclonal antibody

10201-2-AP

AHCY Monoclonal antibody

66019-1-Ig

SHMT2 Polyclonal antibody

11099-1-AP

Gamma Cystathionase Monoclonal antibody

60234-1-Ig

MTR Polyclonal antibody

25896-1-AP