CBS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CBS Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(132)

Kat-Nr. : 14787-1-AP

Synonyme

Beta thionase, Beta-thionase, cystathionine beta synthase, Cystathionine beta-synthase, EC:4.2.1.22

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  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 14787-1-AP

  WB result of CBS antibody (14787-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected NCI-H1299 cells.

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  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14787-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14787-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 14787-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 14787-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14787-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14787-1-AP

  IP result of anti-CBS (IP:14787-1-AP, 3ug; Detection:14787-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14787-1-AP (CBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 14787-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, MCF-7-Zellen, Maus-Kolongewebe, Mausnierengewebe, NCI-H1299-Zellen, Rattenhirngewebe, Rattennierengewebe, Rattenlebergewebe, SKOV-3-Zellen, THP-1-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolongewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 113 publications below
• IHC: See 24 publications below
• IF: See 19 publications below

Produktinformation

14787-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CBS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBS fusion protein Ag6437
• Vollständiger Name: cystathionine-beta-synthase
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61-63 kDa
• GenBank-Zugangsnummer: BC000440
• Gene symbol: CBS
• Gene ID (NCBI): 875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Publikationen

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
canine	IF	Nat Commun; Remote ischemic conditioning counteracts the intestinal damage of necrotizing enterocolitis by improving intestinal microcirculation.; Authors - Yuhki Koike
mouse	WB,IHC,IF	Redox Biol; Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity; Authors - Zhengqiang Yuan
human	WB	Cancer Res; PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer; Authors - Takehiro Yamamoto
mouse	WB	Redox Biol; Cystathionine γ-lyase promotes estrogen-stimulated uterine artery blood flow via glutathione homeostasis.; Authors - Rachael Bok