CBY1 Polyklonaler Antikörper

CBY1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 12239-1-AP

Synonyme

arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1

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• 

  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12239-1-AP

  IP result of anti-CBY1 (IP:12239-1-AP, 4ug; Detection:12239-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12239-1-AP bindet in WB, IHC, IF, IP, ELISA CBY1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBY1 fusion protein Ag2882
• Vollständiger Name: chibby homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 126 aa, 14 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC016139
• Gene symbol: CBY1
• Gene ID (NCBI): 25776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
mouse,human	WB,IF	Dev Dyn; DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.; Authors - Lilong Guo
human	IHC	Medicine (Baltimore); Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma; Authors - Gang Ren
human	IF	Adv Sci (Weinh); The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers; Authors - Huicheng Chen
human	WB	Heliyon; MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling; Authors - Qingqing Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Feng-Qian (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T