SGF29 Polyklonaler Antikörper

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• KD/KO Validated

SGF29 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24061-1-AP

Synonyme

CCDC101, SGF29

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• 

  WB analysis of HeLa using 24061-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 24061-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human liver cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human cervical cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human cervical cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

24061-1-AP bindet in WB, IHC, ELISA SGF29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGF29 fusion protein Ag21261
• Vollständiger Name: coiled-coil domain containing 101
• Berechnetes Molekulargewicht: 293 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC011981
• Gene symbol: SGF29
• Gene ID (NCBI): 112869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

Publikationen

Species	Application	Title
human	WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long ; KO Validated