MCU/CCDC109A Polyklonaler Antikörper

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• KD/KO Validated

MCU/CCDC109A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 26312-1-AP

Synonyme

C10orf42, Calcium uniporter protein, CCDC109A, MCU, MCU/CCDC109A

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Galerie der Validierungsdaten

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  WB analysis using 26312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26312-1-AP

  WB result of MCU/CCDC109A antibody (26312-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCU/CCDC109A transfected HeLa cells.

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  IHC staining of mouse kidney using 26312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

26312-1-AP bindet in WB, IHC, IF, ELISA MCU/CCDC109A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCU/CCDC109A fusion protein Ag24733
• Vollständiger Name: coiled-coil domain containing 109A
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC034235
• Gene symbol: CCDC109A
• Gene ID (NCBI): 90550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCU, also known as CCDC109A, is highly conserved across all eukaryotes. The MCU protein is composed of two coiled-coil domains, two TMDs, and a short motif of amino acids between the two TMDs. Knockdown of MCU dramatically reduces mitochondrial Ca2+ uptake in isolated mitochondria, in permeabilized cells and living cells. MCU has 3 isoforms with MW 40,37 and 35 kDa (refer to UniProt). The observed MW of MCU is mainly between 30 to 35 kDa in paper (PMID: 26341627; 28337252).

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak.; Authors - Giovanni Quarato
human	WB	Cell Rep; A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease; Authors - Vinita Bharat
mouse	WB	Int J Mol Sci; Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes; Authors - Tianyang Lan
human	WB	Cancer Sci; EFHD1, a novel mitochondrial regulator of tumor metastasis in clear cell renal cell carcinoma; Authors - Kun Meng
rat	WB	Mol Nutr Food Res; Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).; Authors - Sicong Tian
rat	WB	Stem Cell Res Ther; PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca2+ efflux.; Authors - Qin Zhou