CEP89, CCDC123 Polyklonaler Antikörper

CEP89, CCDC123 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 24002-1-AP

Synonyme

CCDC123, CEP123, CEP89

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 24002-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 24002-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 24002-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 24002-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 24002-1-AP (CEP89 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IF: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

24002-1-AP bindet in WB, IF, ELISA CEP89, CCDC123 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP89, CCDC123 fusion protein Ag21206
• Vollständiger Name: coiled-coil domain containing 123
• Berechnetes Molekulargewicht: 783 aa, 90 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa, 40 kDa
• GenBank-Zugangsnummer: BC136328
• Gene symbol: CCDC123
• Gene ID (NCBI): 84902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	IF	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	IF	PLoS Biol; The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly; Authors - Pierrick Le Borgne