CCKBR-specific Polyklonaler Antikörper

CCKBR-specific Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16549-1-AP

Synonyme

CCKBR, CCK2-R, CCK2R, CCK2 R, CCK BR

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  WB analysis of BxPC-3 using 16549-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 16549-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 16549-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 16549-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, humanes Magengewebe, SGC-7901-Zellen
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16549-1-AP bindet in WB, IHC, IF/ICC, ELISA CCKBR-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cholecystokinin B receptor
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 64 kDa, 50 kDa
• GenBank-Zugangsnummer: NM_176875
• Gene symbol: CCKBR
• Gene ID (NCBI): 887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

Publikationen

Species	Application	Title
human	WB,IHC	Mol Pharm; Pseudomonas Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.; Authors - Jiang Chang
human	IF	J Cancer Res Clin Oncol; Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target.; Authors - Jiang Chang