CCL1 Rekombinanter Antikörper

CCL1 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250094D6

Kat-Nr. : 98502-2-RR

Synonyme

C-C motif chemokine 1, P500, Scya1, SIS-epsilon, Small-inducible cytokine A1

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  FC experiment of Transfected HEK-293T using 98502-2-RR

  1x10^6 HEK-293T cells or CCL1-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL1 Rabbit RecAb (98502-2-RR, Clone: 250094D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98502-2-RR

  1x10^6 HEK-293T cells or CCL1-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL1 Rabbit RecAb (98502-2-RR, Clone: 250094D6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98502-2-RR bindet in FC (Intra) CCL1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL1 fusion protein Eg3293
• Vollständiger Name: chemokine (C-C motif) ligand 1
• Berechnetes Molekulargewicht: 10KD
• GenBank-Zugangsnummer: NM_011329.3
• Gene symbol: Ccl1
• Gene ID (NCBI): 20290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CCL1, also known as I-309 in human or TCA-3 in mice, is a small glycoprotein belonging to the CC chemokine family. It is predominantly expressed by activated T cells, monocytes, and endothelial cells, and primarily acts as a chemoattractant for monocytes and T cells. CCL1 binds to the receptor CCR8, which is expressed on monocytes, neutrophils, and T cells, including within the thymus. This chemokine may perform dual functions: as an inflammatory chemokine during leukocyte migration into inflammatory sites and as a constitutive chemokine during T cell selection in the thymus. Furthermore, CCL1 has been associated with allergic inflammation and the recruitment of Th2 cells, suggesting a role in allergic diseases such as asthma.