CCL12/MCP-5 Rekombinanter Antikörper

CCL12/MCP-5 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250386G10

Kat-Nr. : 98542-1-RR

Synonyme

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

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  FC experiment of Transfected HEK-293T using 98542-1-RR

  1x10^6 HEK-293T cells and CCL12/MCP-5-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse Ccl12 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98542-1-RR

  1x10^6 HEK-293T cells (left) or CCL12/MCP-5-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL12/MCP-5 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98542-1-RR bindet in FC (Intra) CCL12/MCP-5 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL12/MCP-5 fusion protein Eg3315
• Vollständiger Name: chemokine (C-C motif) ligand 12
• Berechnetes Molekulargewicht: 12KD
• GenBank-Zugangsnummer: NM_011331.2
• Gene symbol: Ccl12
• Gene ID (NCBI): 20293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.