CCL14 Rekombinanter Antikörper

CCL14 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250487E9

Kat-Nr. : 98554-1-RR

Synonyme

C C motif chemokine 14, C-C motif chemokine 14, CCL-14, Chemokine CC-1/CC-3, HCC-1(1-74)

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  FC experiment of Transfected CHO using 98554-1-RR

  1x10^6 CHO cells or CCL14-transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human CCL14 Rabbit RecAb (98554-1-RR, Clone:250487E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected CHO using 98554-1-RR

  1x10^6 CHO cells or CCL14-transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human CCL14 Rabbit RecAb (98554-1-RR, Clone:250487E9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected CHO cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98554-1-RR bindet in FC (Intra) CCL14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL14 fusion protein Eg2879
• Vollständiger Name: chemokine (C-C motif) ligand 14
• Berechnetes Molekulargewicht: 11k kDa
• GenBank-Zugangsnummer: NM_032963.3
• Gene symbol: CCL14
• Gene ID (NCBI): 6358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CCL14, also known as HCC-1, is a human plasma chemokine that belongs to the CC chemokine family. It was originally collected and purified from the hemofiltrate of patients with chronic renal failure. CCL14 is constitutively expressed in various tissues, including the spleen, bone marrow, liver, muscle, and intestine. CCL14 serves as a novel prognostic factor and tumor suppressor of HCC by modulating cell cycle and promoting apoptosis (PMID: 31641099), and CCL14 is a potential biomarker associated with immune cell infiltration in lung adenocarcinoma (PMID: 39030403).