CCL17/TARC Rekombinanter Antikörper

CCL17/TARC Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250385D10

Kat-Nr. : 98553-1-RR

Synonyme

ABCD-2, CC chemokine TARC, C-C motif chemokine 17, Ccl17, Small-inducible cytokine A17

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  FC experiment of Transfected HEK-293T using 98553-1-RR

  1x10^6 HEK-293T cells or CCL17/TARC-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL17/TARC Rabbit RecAb (98553-1-RR, Clone: 250385D10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98553-1-RR

  1x10^6 HEK-293T cells or CCL17/TARC-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL17/TARC Rabbit RecAb (98553-1-RR, Clone: 250385D10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98553-1-RR bindet in FC (Intra) CCL17/TARC und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL17/TARC fusion protein Eg3319
• Vollständiger Name: chemokine (C-C motif) ligand 17
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_011332.3
• Gene symbol: Ccl17
• Gene ID (NCBI): 20295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CCL17, also named as TARC, is a member of the CC chemokine family, and is highly expressed by thymus and other cells, including keratinocytes, endothelial cells, dendritic cells, bronchial epithelial cells and fibroblasts. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 induced chemotaxis and Ca2+ influx in the cells stably expressing CCR4, thereby demonstrating the ligand-receptor relationship between CCL17 and CCR4.