CCL20/MIP-3 alpha Rekombinanter Antikörper

CCL20/MIP-3 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250239E12

Kat-Nr. : 98519-1-RR

Synonyme

Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20

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  FC experiment of Transfected HEK-293T using 98519-1-RR

  1x10^6 HEK-293T cells or CCL20/MIP-3 alpha transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98519-1-RR

  1x10^6 HEK-293T cells or CCL20/MIP-3 alpha-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98519-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98519-1-RR against Mouse CCL20/MIP-3 alpha were performed. The affinity constant is 3.09 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98519-1-RR bindet in FC (Intra) CCL20/MIP-3 alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL20/MIP-3 alpha fusion protein Eg3311
• Vollständiger Name: chemokine (C-C motif) ligand 20
• Berechnetes Molekulargewicht: 11KD
• GenBank-Zugangsnummer: NM_001159738.1
• Gene symbol: Ccl20
• Gene ID (NCBI): 20297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.