CCL20/MIP-3 alpha Rekombinanter Antikörper

CCL20/MIP-3 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Biotin

CloneNo.

241694B2

Kat-Nr. : Biotin-FcA98221

Synonyme

CCL20, MIP-3 Alpha, MIP-3α, Beta-chemokine exodus-1, C C motif chemokine 20

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  FC experiment of THP-1 using Biotin-FcA98221

  1x10^6 untreated or PMA, LPS and Brefeldin A treated THP-1 cells were surface stained with 0.25 ug Biotin Anti-Human CCL20/MIP-3 alpha Rabbit RecAb (Biotin-FcA98221, Clone: 241694B2) and PE Streptavidin (Cat.No. PF00021). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of THP-1 using Biotin-FcA98221

  1x10^6 PMA, LPS and Brefeldin A treated THP-1 cells were surface stained with 0.25 ug Biotin Anti-Human CCL20/MIP-3 alpha Rabbit RecAb (Biotin-FcA98221, Clone: 241694B2) (red) or 0.25 ug FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated cells were surface stained with 0.25 ug Biotin Anti-Human CCL20/MIP-3 alpha Rabbit RecAb (Biotin-FcA98221, Clone: 241694B2) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA, LPS and Brefeldin A treated THP-1 cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-FcA98221 bindet in FC (Intra) CCL20/MIP-3 alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL20/MIP-3 alpha fusion protein Eg2398
• Vollständiger Name: chemokine (C-C motif) ligand 20
• Berechnetes Molekulargewicht: 11kDa
• GenBank-Zugangsnummer: NM_004591.3
• Gene symbol: CCL20/MIP-3 alpha
• Gene ID (NCBI): 6364
• Konjugation: Biotin
• Excitation/Emission maxima wavelengths: -
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.