CCL22/MDC Rekombinanter Antikörper

CCL22/MDC Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250115D3

Kat-Nr. : 98501-2-RR

Synonyme

Abcd1, Activated B and dendritic cell-derived, CC chemokine ABCD-1, C-C motif chemokine 22, Ccl22

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  FC experiment of Transfected HEK-293T using 98501-2-RR

  1x10^6 HEK-293T cells or CCL22/MDC transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL22/MDC Rabbit RecAb (98501-2-RR, Clone: 250115D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98501-2-RR

  1x10^6 HEK-293T cells or CCL22/MDC transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL22/MDC Rabbit RecAb (98501-2-RR, Clone: 250115D3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98501-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98501-2-RR against Mouse CCL22/MDC were performed. The affinity constant is 0.914 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98501-2-RR bindet in FC (Intra) CCL22/MDC und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL22/MDC fusion protein Eg2241
• Vollständiger Name: chemokine (C-C motif) ligand 22
• Berechnetes Molekulargewicht: 10kd
• GenBank-Zugangsnummer: NM_009137
• Gene symbol: CCL22/MDC
• Gene ID (NCBI): 20299
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.