CCL3/MIP-1 alpha Rekombinanter Antikörper

CCL3/MIP-1 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241695B10

Kat-Nr. : 98194-1-RR

Synonyme

241695B10, C-C motif chemokine 3, Ccl3, Heparin-binding chemotaxis protein, L2G25B

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  FC experiment of J774A.1 using 98194-1-RR

  1x10^6 LPS and Brefeldin A treated J774A.1 cells were intracellularly stained with 0.25 ug Anti-Mouse CCL3 Rabbit Recombinant Antibody (98194-1-RR, Clone:241695B10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated J774A.1 cells were intracellularly stained with 0.25 ug Anti-Mouse CCL3 Rabbit Recombinant Antibody (98194-1-RR, Clone: 241695B10) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98194-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98194-1-RR against Mouse CCL3 were performed. The affinity constant is 3.87 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): LPS and Brefeldin A treated J774A.1 cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98194-1-RR bindet in FC (Intra) CCL3/MIP-1 alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL3/MIP-1 alpha fusion protein Eg2170
• Vollständiger Name: chemokine (C-C motif) ligand 3
• Berechnetes Molekulargewicht: 10kd
• GenBank-Zugangsnummer: NM_011337
• Gene symbol: Ccl3
• Gene ID (NCBI): 20302
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.