CCL9 Rekombinanter Antikörper

CCL9 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

243103H5

Kat-Nr. : 98475-1-RR

Synonyme

C-C motif chemokine 9, CCF18, CCL9(29-101), CCL9(30-101), CCL9(31-101)

---

• 

  FC experiment of Transfected HEK-293T using 98475-1-RR

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone: 243103H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98475-1-RR

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone:243103H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98475-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98475-1-RR against Mouse CCL9 were performed. The affinity constant is 0.649 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98475-1-RR bindet in FC (Intra) CCL9 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL9 fusion protein Eg3336
• Vollständiger Name: chemokine (C-C motif) ligand 9
• Berechnetes Molekulargewicht: 14KD
• GenBank-Zugangsnummer: NM_011338.2
• Gene symbol: Ccl9
• Gene ID (NCBI): 20308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Mouse C-C motif ligand 9 (CCL9), alternatively named macrophage inflammatory protein 1γ (MIP-1γ), was identified in 1995 and is homologous to the mouse CCL6, as well as human CCL23 and CCL15. It is noted that CCL9 is also known by various names such as macrophage inflammatory protein-1 gamma (MIP-1ɣ), macrophage inflammatory protein-related protein-2 (MRP-2) and CCF18 in rodents. Monocytes and myeloid cell lines produce large quantities of CCL9, as do dendritic cells and T cells, in particular Th1 type T cells. Despite high baseline levels in circulation, it has become apparent that concentrations of CCL9 vary greatly in specific tissues with profound effects on health. For example, in the bone, CCL9 is produced at even higher levels, and is critical to osteoclast versus osteoblast differentiation of macrophages. There are also indications of a timed specific induction of CCL9 in skin wound healing and follicle-associated epithelium of the gut.