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Cyclin B1 Polyklonaler Antikörper
Cyclin B1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (3)
Anwendung
WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 55004-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, PC-3-Zellen | 
| Erfolgreiche IP | HeLa-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Hautkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, U2OS-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:200-1:800 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 272 publications below | 
| IF | See 3 publications below | 
| IP | See 1 publications below | 
| CoIP | See 2 publications below | 
| ChIP | See 1 publications below | 
Produktinformation
55004-1-AP bindet in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA Cyclin B1 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte, Rind | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | Peptid | 
| Vollständiger Name | cyclin B1 | 
| Berechnetes Molekulargewicht | 48 kDa | 
| Beobachtetes Molekulargewicht | 55-60 kDa | 
| GenBank-Zugangsnummer | NM_031966 | 
| Gene symbol | Cyclin B1 | 
| Gene ID (NCBI) | 891 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Cyclin B1 antibody 55004-1-AP | Protokoll herunterladen | 
| IHC protocol for Cyclin B1 antibody 55004-1-AP | Protokoll herunterladenl | 
| IF protocol for Cyclin B1 antibody 55004-1-AP | Protokoll herunterladen | 
| IP protocol for Cyclin B1 antibody 55004-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration | ||
| Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis | ||
| Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
| Acta Pharm Sin B Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway. | ||
| Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Tsimafei (Verified Customer) (08-30-2025) | Detection of Cyclin B1 in human U2OS cells. Good antibody! 
  | 
| FH Aleksandr (Verified Customer) (07-29-2025) | Excellent antibody 
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