Cyclin D1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Cyclin D1 Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(823)

CloneNo.

2G3G5

Rezensionen (3)

Kat-Nr. : 60186-1-Ig

Synonyme

CCND1, 2G3G5, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL-1

---

• 

  WB analysis using 60186-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60186-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60186-1-Ig

  WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells.

• 

  WB analysis using 60186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 60186-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60186-1-Ig

  Western blot of Cyclin D1 in HepG2, SW 1990 and NIH/3T3 cell lines with 60186-1-Ig at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 60186-1-Ig

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Cyclin D1 Monoclonal antibody (60186-1-Ig, Clone:2G3G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A431-Zellen, A549-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen, SW 1990-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 795 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• IP: See 4 publications below

Produktinformation

60186-1-Ig bindet in WB, IF, FC (Intra), IP, ELISA Cyclin D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Kaninchen, Maus, Ratte, Rind, Zebrafisch, Ziege
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin D1 fusion protein Ag0689
• Vollständiger Name: cyclin D1
• Berechnetes Molekulargewicht: 295 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000076
• Gene symbol: Cyclin D1
• Gene ID (NCBI): 595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.; Authors - Guixin Zhu
human	WB	Bioact Mater; Bioinspired engineering ADSC nanovesicles thermosensitive hydrogel enhance autophagy of dermal papilla cells for androgenetic alopecia treatment; Authors - Jiachao Xiong
human	IP	Nat Commun; F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression; Authors - Feng Li
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
mouse	WB	Adv Sci (Weinh); Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis; Authors - Bin Liu
mouse	WB	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Janardhan (Verified Customer) (08-22-2020)

Antibody works ok, needs longer exposure

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Medulloblastoma

FH

Yuan (Verified Customer) (01-23-2020)

The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hela cell

FH

Kyosuke (Verified Customer) (06-12-2019)

I use this as a proliferation marker and this antibody works very well for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: rat aortic smooth muscle cells