Cyclin D2 Polyklonaler Antikörper

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• KD/KO Validated

Cyclin D2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

46

Kat-Nr. : 10934-1-AP

Synonyme

CCND2, cyclin D, cyclin D2, G1/S-specific cyclin-D2

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Galerie der Validierungsdaten

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  WB analysis using 10934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10934-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10934-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10934-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10934-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 38 publications below

Produktinformation

10934-1-AP bindet in WB, IHC, ELISA Cyclin D2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin D2 fusion protein Ag1377
• Vollständiger Name: cyclin D2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC010958
• Gene symbol: CCND2
• Gene ID (NCBI): 894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Publikationen

Species	Application	Title
mouse		Nature; SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.; Authors - Soufiane Boumahdi
human	WB	Cancer Cell; The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.; Authors - Natalia Martinez-Soria ; KD Validated
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
mouse	WB	Dev Cell; Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.; Authors - Yan Gong
mouse		Kidney Int; Transcription factor MafB may play an important role in secondary hyperparathyroidism.; Authors - Naoki Morito
human	WB	Int J Cancer; Human tumor microRNA signatures derived from large-scale oligonucleotide microarray datasets.; Authors - Wang Wenzhang W

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