Cyclin D2 Monoklonaler Antikörper

Cyclin D2 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1A12A8

Kat-Nr. : 67048-1-Ig

Synonyme

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002

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Galerie der Validierungsdaten

• 

  WB analysis using 67048-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67048-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Caco-2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

67048-1-Ig bindet in WB, ELISA Cyclin D2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin D2 fusion protein Ag25190
• Vollständiger Name: cyclin D2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC010958
• Gene symbol: CCND2
• Gene ID (NCBI): 894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Publikationen

Species	Application	Title
human	WB	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma
human	WB	Technol Cancer Res Treat; The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells.; Authors - AiJun Huang
human	WB	Cell Death Dis; Long intergenic non-coding RNA 00473 promotes proliferation and migration of gastric cancer via the miR-16-5p/CCND2 axis and by regulating AQP3.; Authors - Shuaishuai Zhuo
human,mouse	WB	Am J Cancer Res; miR-1297 inhibits osteosarcoma cell proliferation and growth by targeting CCND2; Authors - Pan Jiang