Cyclin E2 Polyklonaler Antikörper

Cyclin E2 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(60)

Rezensionen (1)

Kat-Nr. : 11935-1-AP

Synonyme

CCNE2, CYCE2, Cyclin E, G1/S specific cyclin E2, G1/S-specific cyclin-E2

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  IP experiment of Jurkat using 11935-1-AP

  IP result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug.

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  IHC staining of human breast cancer using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E2 antibody (11935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 11935-1-AP

  Immunofluorescent analysis of HeLa cells using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 11935-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E2 Polyclonal antibody (11935-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 55 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

11935-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Cyclin E2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin E2 fusion protein Ag2532
• Vollständiger Name: cyclin E2
• Berechnetes Molekulargewicht: 374 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC020729
• Gene symbol: CCNE2
• Gene ID (NCBI): 9134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration; Authors - Dongcheng Cai
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
mouse	IHC	Theranostics; Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction; Authors - Meng Gao
mouse,human	WB	Cell Commun Signal; Decursin induces FLT3-ITD acute myeloid leukemia cell apoptosis by increasing the expression of the ubiquitin-conjugase UBE2L6; Authors - Tianxin Zhang
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juri (Verified Customer) (12-22-2023)

Worked ok with immunocytochemistry

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 200
• Cell Tissue Type: Cell