Cyclin E2 Polyklonaler Antikörper

Cyclin E2 Polyklonal Antikörper für FC, IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Rezensionen (1)

Kat-Nr. : 11935-1-AP

Synonyme

CCNE2, CYCE2, Cyclin E, cyclin E2, G1/S specific cyclin E2

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Galerie der Validierungsdaten

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  IP experiment of Jurkat using 11935-1-AP

  IP Result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E2 antibody (11935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 11935-1-AP

  Immunofluorescent analysis of HeLa cells using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HeLa using 11935-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cyclin E2 antibody (11935-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 38 publications below
• IHC: See 3 publications below

Produktinformation

11935-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Cyclin E2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin E2 fusion protein Ag2532
• Vollständiger Name: cyclin E2
• Berechnetes Molekulargewicht: 374 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC020729
• Gene symbol: CCNE2
• Gene ID (NCBI): 9134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui
human	WB	Mol Ther Nucleic Acids; lncRNA STEAP3-AS1 Modulates Cell Cycle Progression via Affecting CDKN1C Expression through STEAP3 in Colon Cancer.; Authors - Heya Na
human	WB	Oncotarget; Upregulation of E2F8 promotes cell proliferation and tumorigenicity in breast cancer by modulating G1/S phase transition.; Authors - Liping Ye
human	WB, IHC	Cancers (Basel); A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.; Authors - Chiao-Yun Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Juri (Verified Customer) (12-22-2023)

Worked ok with immunocytochemistry

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 200
• Cell Tissue Type: Cell