Cyclin H Polyklonaler Antikörper

Cyclin H Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10718-1-AP

Synonyme

CAK, CCNH, cyclin H, MO15 associated protein, p34, p37

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Galerie der Validierungsdaten

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  WB analysis using 10718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 10718-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10718-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 10718-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10718-1-AP

  IP Result of anti-Cyclin H (IP:10718-1-AP, 4ug; Detection:10718-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human breast cancer using 10718-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10718-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10718-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using CCNH antibody 10718-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of SH-SY5Y using 10718-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Cyclin H antibody (10718-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, HeLa-Zellen, HuH-7-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10718-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Cyclin H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin H fusion protein Ag1088
• Vollständiger Name: cyclin H
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC005280
• Gene symbol: CCNH
• Gene ID (NCBI): 902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.