cyclin I Polyklonaler Antikörper

cyclin I Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16357-1-AP

Synonyme

CCNI, Cyclin-I

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  WB analysis using 16357-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16357-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 16357-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 16357-1-AP (cyclin I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16357-1-AP (cyclin I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16357-1-AP (cyclin I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16357-1-AP (cyclin I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16357-1-AP bindet in WB, IHC, ELISA cyclin I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: cyclin I fusion protein Ag0493
• Vollständiger Name: cyclin I
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC004975
• Gene symbol: CCNI
• Gene ID (NCBI): 10983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.