CCS Polyklonaler Antikörper

CCS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 22802-1-AP

Synonyme

CCS, SOD 4, SOD4, Superoxide Dismutase 4

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 22802-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22802-1-AP (CCS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 22802-1-AP

  IP Result of anti-CCS (IP:22802-1-AP, 3ug; Detection:22802-1-AP 1:1000) with HepG2 cells lysate 6000ug.

• 

  IHC staining of mouse brain using 22802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22802-1-AP (CCS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22802-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22802-1-AP (CCS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22802-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22802-1-AP (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22802-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 22802-1-AP (CCS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

22802-1-AP bindet in WB, IP, IHC, IF, ELISA CCS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCS fusion protein Ag18850
• Vollständiger Name: copper chaperone for superoxide dismutase
• Berechnetes Molekulargewicht: 274 aa, 29 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC105016
• Gene symbol: CCS
• Gene ID (NCBI): 9973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
human	WB	Neurobiol Dis; The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.; Authors - Nadine Bakkar
mouse	WB	Sci Rep; S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.; Authors - Sarah E Antinone
human	WB	Br J Cancer; AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy; Authors - Xiaomei Zhang
mouse	WB	Mol Med Rep; Artemether ameliorates adriamycin induced cardiac atrophy in mice; Authors - Wenci Weng