CD103 Monoklonaler Antikörper

CD103 Monoklonal Antikörper für FC

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2E7

Kat-Nr. : 65047-1-Ig

Synonyme

A530055J10, alpha E1, alpha M290, aM290, CD103, Integrin alpha E, Integrin alpha M290, Itgae

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  FC experiment of C57BL/6 mouse splenocytes using 65047-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD103 (65047-1-Ig, Clone:2E7) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100, or 0.5 ug Armenian Hamster IgG isotype control and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

65047-1-Ig bindet in IF, FC CD103 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/6J Maus Intraepitheliale Lymphozyten (IEL)
• Vollständiger Name: integrin alpha E, epithelial-associated
• GenBank-Zugangsnummer: BC150690
• Gene symbol: CD103
• Gene ID (NCBI): 16407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

Publikationen

Species	Application	Title
mouse	IF	Biomolecules; Oral Immunization with Escherichia coli Nissle 1917 Expressing SARS-CoV-2 Spike Protein Induces Mucosal and Systemic Antibody Responses in Mice; Authors - Giovanni Sarnelli
mouse	FC	J Nanobiotechnology; Optogenetic engineered umbilical cord MSC-derived exosomes for remodeling of the immune microenvironment in diabetic wounds and the promotion of tissue repair; Authors - Xin Zhao
	IF	Biomed Pharmacother; Intranasal administration of Escherichia coli Nissle expressing the spike protein of SARS-CoV-2 induces long-term immunization and prevents spike protein-mediated lung injury in mice; Authors - Giovanni Sarnelli