CD106 Monoklonaler Antikörper

CD106 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Fluorescent Dye

Publikationen(1)

CloneNo.

1.G11B1

Kat-Nr. : FITC-65049

Synonyme

VCAM1, VCAM-1, V CAM 1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM

---

• 

  FC experiment of HUVEC using FITC-65049

  1X10^6 TNF-alpha treated HUVEC cells were surface stained with 10 ul FITC Anti-Human CD106 (FITC-65049, Clone: 1.G11B1) (green) or FITC-Mouse IgG1 isotype control (black). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

FITC-65049 bindet in FC CD106 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 739 aa, 81 kDa
• GenBank-Zugangsnummer: BC017276
• Gene symbol: VCAM-1
• Gene ID (NCBI): 7412
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publikationen

Species	Application	Title
human	FC	J Physiol Biochem; Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues; Authors - Bairong Fang